Biorepository

***The CALERIETM sample repositories have been relocated to the NIA. *** Please note that the samples are retained at the NIA Aging Research Biobank and the numbers below reflect sample numbers at the time of transfer to the NIA Aging Research Biobank. Please confirm sample availability with NIA Aging Research Biobank.


The main CALERIETM protocol called for samples of blood, urine, and tissue to be procured for repository storage in the following quantities: 

Sample type

Visits at baseline, 12 months
and 24 months

Visits at 3 months, 6 months, and 18
months

Serum 10 ml 4 ml
Serum (OGTT)4 ml  
Plasma (EDTA) *9 ml4 ml
Plasma (citrate)1 ml 
RNAExtracted from whole blood at BL, 12 months and 24 months 
DNAExtracted from whole blood at BL, 12 months and 24 months 
Urine (no additive)18 ml  
Urine (with boric acid)18 ml  
Skeletal muscle~ 45 individualsNote: a small subset of CALERIE individuals had all three samples
Adipose tissue~ 45 individualsNote: a small subset of CALERIE individuals had all three samples

Some of the aliquotting of samples was be performed at the field centers; the remainder occurred upon arrival at our laboratory.  Regardless of location, the personnel entrusted with this responsibility were appropriately trained and demonstrated competence in the application of the aliquotting procedure.  Most of the aliquots for storage measured either 0.5 ml or 1.0 ml volumes.  

* Note: some baseline, 12-month; and 24-month arterialized plasma samples were collected from a hand vein using a hot-box chamber.  These samples were used only for determination of plasma catecholamine concentrations and were not used for any other purpose.  None of these samples remain in the biorepository.  All other samples used for other analyses and for future use were collected under standard conditions.

 

CALERIETM SAMPLES: Numbers of participants with at least one stored sample of the type and at the times indicated.

Sample Type       Baseline3 month6 month12 month18 month24 month
Serum207201196188178180
Serum (OGTT)205  184 176
Plasma (EDTA)207201196187182180
Plasma (citrate)207  188 180
RNA207  189 180
DNA207  188 180
Urine (no additive)208  187 180
Urine (boric acid)208  187 180
Skeletal muscle samples88  43 30
Skeletal muscle IHC88  43 30
Skeletal muscle RNA91  43 31
Adipose tissue samples80  33 16
Adipose tissue IHC84  39 16
Adipose tissue RNA92  43 

28

 

OGTT = Baseline sample from oral glucose tolerance test; IHC = tissue/slides prepared for immunohistochemistry

TM